Diario del páncreas Acceso abierto

Abstracto

Additive Effects of Resveratrol and Resveratrol/Quercetin in Prevention of Hyperglycemia-Mediated Cell Death through Downregulation of NADPH Oxidase and RAGE Expression

Mahboob Zahra, Hemmati Mina, Khorashadizadeh Mohsen, Gholami Maryam

Objective Oxidative stress has been postulated to be a significant mechanism in the progression of diabetic nephropathy. Methods The cytoprotective effects of resveratrol, quercetin and the mixture of RSV/Q in high glucose-treated HEK-293 cells was evaluated by lactate dehydrogenase release and MTT assay. The levels of gene expression of nicotinamide adenine dinucleotide phosphate hydrogen oxidase as the main source of reactive oxygen species generation, RAGE, and SMP30 in high glucose media with or without RSV, Q, or RSV/Q were estimated by real-time PCR. The results showed that RSV, Q, and especially RSV/Q increased cell viability in a dose-dependent manner. Gene expression also showed that RSV, Q, and notably RSV/Q treatment attenuated nicotinamide adenine dinucleotide phosphate hydrogen oxidase and RAGE expression and improved SMP30 levels when compared to high glucose medium alone. Conclusion It was concluded that RSV, Q, and RSV/Q effectively attenuate hyperglycemia-mediated cytotoxicity in HEK-293 cells and should be considered a new therapeutic strategy for the treatment of diabetic nephropathy.