Revista Europea de Biología Experimental Acceso abierto

Abstracto

Callus induction in Alstroemeria using NAA and BAP

Seyyed Rahim Seyyedyousefi, Behzad Kaviani, Naghi Padasht Dehkaei and Ali Salehzadeh

This study evaluates the effect of explants type and plant growth regulators (NAA and BAP) on callus formation of Alstroemeria cv. Fuego, an ornamental plant. Alstroemeria uses as cut flower as well as pot and garden plants because of their beautiful attractive flowers with wide variations in flower color. Results showed that the explants source and different concentrations of growth regulators influenced on callus production. Segments of nodes and internodes were cultured in MS basal medium with different concentrations of BAP (0.0 and 0.5 mg l-1) and NAA (0.0, 1.0 and 2.0 mg l-1) to produce callus. Based on the results, node was better explant than internode to produce callus. 0.5 mg l-1 of BAP and 2.0 mg l-1 of NAA induced more callus on the explants