Revista Europea de Biología Experimental Acceso abierto

Abstracto

Designing a H₂O₂ Biosensor by using of Modified Graphite Electrode with Nano-composite of nafion-nile blue-peroxidase Enzyme

A. Kalantar-Dehnavi, S. Rezaei-Zarchi, Gh. Mazaheri, M. Negahdary, R. Malekzadeh and M. Mazdapour

In this research project, we studied nafion-nile blue mediator (catalyzing oxidation reaction and protein revival) nanopolymer composite and made it possible to see redox metaloproteins electrochemically by amending electrode surface, a process that may be helpful in bio-electrochemical applications. In this project, first, electrode surface was amended using nafion-nile blue polymer composite and then the structure of the nanocomposite was studied using cycling voltammetry, differential pulse voltammetry (DPV) and electron microscope. The findings at this level confirmed the formation of nafion-nile blue nanoparticles (70 nm). Next, peroxidase enzyme was stabilized, hydrogen peroxide, as the substrate of this enzyme, was measured, and a biosensor with 0.28 μM sensitivity was introduced.