Revista Europea de Biología Experimental Acceso abierto

Abstracto

Measurement of pesticides using ultraviolet visible spectrophotometer

Sara Zamani

The amount of pesticides in agricultural products has been measured with different methods. In this remaining tests Picloram herbicides in real samples. Spectroscopy method UV-Vis, was measured by Dey Zvtasyvn discontinuous method. To this case that NH2 groups in the meta position (relative to the functional group COOH) in herbicide Picloram by sodium nitrite with hydrochloric acid, turned to salt Dey AZvnyvm and then with the help reagent Beta Nftvl were obtained combination color with Dey Azo, (bottom figure). Absorb in the wavelength was measured 405 nm and curve linear calibration was obtained in the concentration range 15 ppm - 1ppm. Detection limit 0.148 ppm is obtained small extent of 0.495 ppm and percent recovery for example, corn and barley, 100% and for pesticide formulation 102%. Results obtained indicative this is that with simple method cheap but meanwhile practical spectroscopy UV-Vis can be measured amount of insignificant the herbicide in the real samples.