Revista Europea de Biología Experimental Acceso abierto

Abstracto

Screening and characterization of putative antibiotic compound using PCR

Sara Kasim Younis, Sreekanth P. M. and Sudhakar Malla

Although thousands of antibiotics have been isolated from Streptomyces, these represent only a small part of the repertoire of bioactive compounds so far produced. Therefore, isolation of new Streptomyces from natural resources and characterization of their secondary metabolites is a valuable endeavor. Majority of the actinomycetes in soil that are potential drug sources remain uncultivable, and therefore inaccessible for novel antibiotic discovery. Hence there is need to rediscover new drugs active against these drug resistance pathogens. The main objective of the present study was isolation, purification, and characterization of actinomycetes from soil samples, having antimicrobial activity against Bacillus strains. Soil samples were taken from the bank of the Ulsoor lake, Bangalore, India. These samples were serially diluted and plated on actinomycete isolation agar media. Potential colonies were screened, purified, and stored in glycerol stock. Isolates were morphologically and biochemically characterized. Genomic DNA was extracted from the identified isolate, and analyzed using 16s rRNA sequencing. The sequence analysis revealed of the strain to be Streptomyces carpaticus. The culture isolate was grown in the production medium and then isolated with the antibiotic compound. The compound isolated was tested for the antibacterial activity using the tube method and Well plate method. The compound showed high potential of antibacterial activity and the activity is dose dependent.