Revista Europea de Biología Experimental Acceso abierto

Abstracto

Toxicity of low dose TCDD to body weight and glucose-6-phosphatase in liver and kidney cells of mice

Jyoti Jigyasi and Rahul Kundu

Present study communicates the dose and exposure duration dependent effects of very low environmentally available doses of TCDD to the body weight and glucose-6-phosphatase in the liver and kidney cells of mice. The study was designed to test three hypotheses viz. a) TCDD effects body weight by reducing the activity of glucose-6- phosphatse, b) TCDD causes dose and duration dependent effects on glucose-6-phosphatase and, c) The liver will be more effected than the kidney since, the gluconeogenesis mainly occurs in liver. To test the formulated hypotheses groups of female Swiss albino mice were administered different doses of TCDD (0.004 mg/kg bw/d & 0.04 mg/kg bw/d) for 2, 4 and 6 days of exposure durations. The doses were selected according to the LD₅₀ of TCDD to mice and minimum required exposed dose of TCDD to human being through different environmental sources. The results revealed a clear exposure duration dependent effects of TCDD to selected enzyme and body weight. The enzyme activity was more affected in liver than the kidney hampering different metabolic processes in liver cells. The results suggest that binding of TCDD to the AhR may be one of the possibilities that affect different metabolic pathway and body weight of mice.